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Beilstein J. Org. Chem. 2021, 17, 1476–1480, doi:10.3762/bjoc.17.103
Graphical Abstract
Figure 1: Structure of TDA and TEO, the crystallization hosts used for co-crystallization.
Figure 2: Details of the X-ray crystal structures obtained, shown as ORTEP plots at 50% probability, with van...
Figure 3: Possible states of two organic compounds capable of crystallizing at room temperature in a solvent....
Beilstein J. Org. Chem. 2018, 14, 603–617, doi:10.3762/bjoc.14.47
Figure 1: Enzyme-free template-directed extension of an RNA primer by one nucleotide. B = nucleobase, LG = le...
Figure 2: Oligomerization of the 2-methylimidazolide of guanosine-5'-monophosphate on a poly(C) template.
Figure 3: Structures of backbone linkages produced in enzyme-free primer extension reactions: the phosphorami...
Figure 4: System used for studying the template effect with all 64 possible triplets at the extension site (B...
Figure 5: Interactions attracting the incoming nucleotide to the extension site. Besides base pairing via hyd...
Figure 6: Three possible fates of activated nucleotides in aqueous buffer that result from hydrolysis, primer...
Figure 7: Steps and equilibria considered in our quantitative model of chemical primer extension [34]. The model ...
Figure 8: Binding equilibrium between mononucleotides and hairpins representing primer–template duplexes, as ...
Figure 9: Template-directed primer extension on an RNA template performed with OAt-GMP at 1.8 mM (orange), 3....
Figure 10: Copying of four nucleotides on an immobilized RNA duplex, as reported by Deck et al. [32].
Figure 11: Extension cycle of aminoterminal primer with N-protected nucleotides on solid support, as described...
Figure 12: Formation of a highly reactive methylimidazolium bisphosphate from methylimidazolides of nucleotide...
Figure 13: 31P NMR spectrum (161.9 MHz) of crude MeIm-GMP in D2O. The resonance of the imidazolium bisphosphat...
Figure 14: Imidazolium bisphosphate as intermediate in the primer extension reaction, as described by Szostak ...
Figure 15: Proposed steps of enzyme-free primer extension with in situ activation, using the "general condensa...